Joseph Cardiello
Visiting Assistant Professor
Faculty - Block Visitor
Chemistry & Biochemistry
Joe’s biography and research interests:
Joe Cardiello has spent the last decade working on biochemical and biological research involving species ranging from mosses to mammals, and finally to salamanders.
The central theme of Joe’s research projects has been a focus on analyzing proteins or gene expression patterns present during cellular stress responses. During his PhD at CU Boulder, he used ChIP-seq to study how RNA Polymerase II occupancy across the genome changes in response to acute heat shock in mouse fibroblasts. Joe then received a Sie Foundation Fellowship to spend 2 years at the BioFrontiers Institute studying the impact of Down syndrome on primary transcriptional responses to stress. During the end of this first postdoc, he also worked part time in the next generation sequencing facility, handling sample preparation and sequencing projects for other labs, and consulting on next generation sequencing (NGS) based projects. He then completed a second postdoc position abroad in Lund, Sweden working with applying single cell RNA-sequencing to study limb regeneration in salamanders.
Throughout his research projects, Joe has worked primarily as a research generalist: involving himself in the lab from collecting cells or animals through preparing NGS libraries and analyzing the data with a variety of bioinformatic packages and custom analyses.
Joe is a big believer that his research has been strengthened by his biochemical background, enabling him to embrace facets of research projects that were heavy on chemistry or quantitative analyses.
If you have an interest in stress responses in mammalian cells, the effect of trisomy on gene expression, single cell RNA sequencing, or limb regeneration in salamanders, I always love discussing my past and present research.
Selected Publications:
- Cardiello, J., Joven Araus, A., Giatrellis, S., Helsens, C., Simon, A., Leigh, N. Evaluation of genetic demultiplexing of single-cell sequencing data from model species. Life Science Alliance. doi: https://doi.org/10.26508/lsa.202301979
- Cardiello, JF., Westfall, J., Dowell, RD., Allen, MA. Characterizing primary transcriptional responses to short term heat shock in paired fraternal lymphoblastoid lines with and without Down syndrome. Posted on Biorxiv January 2023. doi: https://doi.org/10.1101/2023.01.17.524431
- Cardiello JF., Sanchez GJ., Allen MA., Dowell RD. Lessons from eRNAs: understanding transcriptional regulation through the lens of nascent RNAs. Transcription. 2020. doi: 10.1080/21541264.2019.1704128
- Cardiello J., Goodrich J., Kugel J. Heat shock causes a reversible increase in RNA polymerase II occupancy downstream of mRNA genes, consistent with a global loss in transcriptional termination. Molecular Cellular Biology. 2018. doi: 10.1128/MCB.00181-18.
- Lantz A., Cardiello J., Gee T., Richards M., Rosenstiel T., Fisher A. Biochemical characterization of an isoprene synthase from Campylopus introflexus (heath star moss). Plant Physiology and Biochemistry. 2015. doi: 10.1016/j.plaphy.2015.06.008